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Contributed By Suresh
This article provides a comprehensive exploration of the intricate mechanism behind the separation of components using High-Performance Liquid Chromatography (HPLC). HPLC is a powerful analytical technique widely used in various industries, particularly in pharmaceuticals and chemistry. The article delves into the principles governing the separation process, covering factors such as stationary phases, mobile phases, and detector interactions. Whether you're a researcher, analyst, or enthusiast, this guide offers valuable insights into the inner workings of HPLC, empowering readers to make informed decisions in analytical science.
Contributed By Radhika Mohan B
The void volume in High-Performance Liquid Chromatography (HPLC) columns plays a critical role in separation processes. This article provides an in-depth exploration of HPLC column void volume, discussing its significance, calculation methods, and impact on chromatographic performance. Dive into the intricacies of chromatography, offering valuable insights for chromatographers, researchers, and those aiming for enhanced precision in analytical techniques.
Contributed By Vasundhra R
High-Performance Liquid Chromatography (HPLC) is a cornerstone in analytical chemistry, and the detector plays a pivotal role in the precision of analyses. This article explores the diverse landscape of HPLC detectors, shedding light on various types and their unique functionalities. From UV-Vis detectors to fluorescence and mass spectrometry, delve into the intricacies of each, providing insights for researchers, analysts, and enthusiasts in the field.
Contributed By Vidyasagar K
High-Performance Liquid Chromatography (HPLC) is a cornerstone in pharmaceutical analysis, demanding precision and expertise. This article presents indispensable tips for optimizing HPLC analysis in pharmaceuticals. Covering aspects from sample preparation to column selection, delve into practical insights for pharmaceutical professionals, researchers, and those aiming for excellence in chromatographic analysis.